Journal: International Journal of Molecular Sciences

Article Title: Na + /K + - and Mg 2+ -ATPases and Their Interaction with AMPA, NMDA and D 2 Dopamine Receptors in an Animal Model of Febrile Seizures

doi: 10.3390/ijms232314638

Figure Lengend Snippet: Immunoblotting analysis of alpha subunit of Na + /K + -ATPase ( A ) and dopamine D 2 receptor ( B ) in cortical plasma membranes from rats 5 and 20 days after HIS. Plasma membranes (40 μg) were subjected to SDS-PAGE, electrophoretically transferred to nitrocellulose and probed with anti-alpha 1 Sodium Potassium ATPase and anti-Dopamine D 2 receptor antibody antisera, as described in Materials and Methods. Data, expressed as arbitrary units, are means ± SEM of experiments performed with five different plasma membrane preparations .

Article Snippet: Immunodetection was carried out by incubating the nitrocellulose membranes with the corresponding primary antibody anti-alpha 1 Sodium Potassium ATPase (1:5000, ab7671 from Abcam), anti-NMDAR1 (1:1000, ab134308 from Abcam), recombinant Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (1:1000, ab183797 from Abcam) and Anti-Dopamine D 2 Receptor antibody (1:1000, ab88074 from Abcam). β-Actin antibody (1:5000, ab8226 from Abcam) and Ponceau red staining were used as a gel loading controls.

Techniques: Western Blot, SDS Page

Journal: International Journal of Molecular Sciences

Article Title: Na + /K + - and Mg 2+ -ATPases and Their Interaction with AMPA, NMDA and D 2 Dopamine Receptors in an Animal Model of Febrile Seizures

doi: 10.3390/ijms232314638

Figure Lengend Snippet: Immunoblotting analysis of alpha subunit of GluR1 ( A ) and dopamine D 2 receptor ( B ) subunits in cortical plasma membranes from 2-month-old rats. Plasma membranes (40 μg) were subjected to SDS-PAGE, electrophoretically transferred to nitrocellulose and probed with recombinant anti-Glutamate Receptor 1 antibody and anti-Dopamine D 2 receptor antibody antisera, as described in Materials and Methods. Data, expressed as arbitrary units, are means ± SEM of experiments performed with five different plasma membrane preparations .

Article Snippet: Immunodetection was carried out by incubating the nitrocellulose membranes with the corresponding primary antibody anti-alpha 1 Sodium Potassium ATPase (1:5000, ab7671 from Abcam), anti-NMDAR1 (1:1000, ab134308 from Abcam), recombinant Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (1:1000, ab183797 from Abcam) and Anti-Dopamine D 2 Receptor antibody (1:1000, ab88074 from Abcam). β-Actin antibody (1:5000, ab8226 from Abcam) and Ponceau red staining were used as a gel loading controls.

Techniques: Western Blot, SDS Page, Recombinant